Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Does Tylenol Cause Autism? A Review of the Science Linking Tylenol and Autism Spectrum Disorder AboutLawsuits.com Acetaminophen or Tylenol Toxicity Lect 19 Pharmacology Frontiers Herbal Therapy for the Treatment of Acetaminophen Associated Liver Injury: Recent Advances and Future Perspectives